ABI3730xl | 454FLX Titanium | Illumina GAIIx* | HiSeq2000 | Solid 4 | Helicos | SMRT | |
---|---|---|---|---|---|---|---|
Read length | 900 base | Avg. 400 base Mode 500 base | 2 x 100 base | 2 x 100 base | 2 x 50 base | Avg. 30-35 base Range 25-50 base | Avg. 1000 base Max. 3000 base |
Future RL | 1000 base | 150 base* | ? | 100 base* | ? | ||
Total tags/run | 96 | 1 M | 225-250 M | - | 1.4 B | 600-800 M | |
Total bases/run | 86.4 k | 0.4-0.6 G | 45-50 G | 150-200 G | 80-100 G | 21-28 G | |
Running time | 3 hours | 10 hours | 9.5 days | 8 days | 12-16 days | 8 days | |
Throughput (base) | 30 k/h** | 60 M/h** | 220 M/h** | 1 G/h | 350 M/h** | 140 M/h | 180 G/h? |
Accuracy | 99% | 98% | ? | 99.94% | 95% | ||
Poly N | Max. 12base | ||||||
Cost/instrument*** | $500,000 | $540,000 \88,000,000 | ? | $595,000 \78,000,000 (Solid3) | $999,000 | <||
Cost/Mbase**** | ~$60 | ~$2 | - | ~$2 (Solid3) | ~$1 | ||
ABI3730xl | 454FLX Titanium | Illumina GAIIx* | HiSeq2000 | Solid 4 | Helicos | SMRT |
* link to PDF file
** calculate from catalog spec.
*** Nat. Rev. Genet., 11, 31-46 (2010)
**** Nat. Biotech., 26(10), 1135-1145 (2008)